Pierce bca protein assay kit, sufficient reagents for 500 testtube or 5000 microplate. Pierce bca protein assay kit thermo fisher scientific. Procedure for the pierce microplate bca protein assay kitreducing agent compatible. The table serves as a guide for assessing which protein assay s might be most effective with a given sample type. Pierce offers seven colorimetric assays for detection and quantitation of total protein. The thermo scientific pierce bca protein assay kit is a detergentcompatible formulation based on bicinchoninic acid bca for the colorimetric detection and quantitation of total protein. Thermo scientific pierce protein assay technical handbook. Bradford assay principle explanation this lecture explains about the bradford assay to determine the total protein concentration in a cell. Every protein assay has limitations depending on the application and the specific protein sample analyzed. Bca protein assay reducing agent compatible standard tube 1252,000 gml bca protein assay standard tube or microplate 202,000 gml enhanced tube 5250 gml micro bca protein assay standard tube 0.
They are all wellcharacterized, robust assays that provide consistent, reliable results. Tools and reagents for improved quantitation of total or. Pierce bca protein assay kit is a twocomponent, highprecision, detergentcompatible. Pierce bca protein assays have a unique advantage over the coomassie dyebased assays bradfordthey are compatible with samples that contain up to 5% surfactants detergents, and are affected much less by protein compositional differences, providing greater protein to protein uniformity. Pierce bca protein assay kit university of arizona. Measuring protein concentration using the bca protein assay with. Bsa bovine serum albumin is used as the standard and samples are. This is the protocol to be used in the determination of total protein in brain tissue by the pierce bca method. Pierce microplate bca protein assay kit fisher scientific. The pierce bca protein assay is a detergentcompatible formulation based on bicinchoninic acid bca for. The bca protein assay reducing agent compatible standard protocol. We did the pierce bca assay and ran the standard curve and were able to estimate protein concentrations in our samples. If precise quantitation of an unknown protein is required, it is advisable to select a protein.
Results of these compatibility experiments are summarized in tabular form on the following two pages. However, because this method is laborious and typically yields. Piercea protein assay kit bc thermo fisher scientific. The glomax discover system and pierce bca protein assay provide a simple method for quantifying protein over a large range. Greater protein to protein variability than the bca assay high levels of ionic detergents require the addition of the ionic detergent compatibility reagent idcr. The most common protein assay reagents involve either protein dye binding chemistry coomassiebradford or protein copper chelation chemistry biuret bca. Thermo scientific pierce 660 nm protein assay overview highlights typical response curves 14 bca based protein assays 15 chemistry of the bca protein assay 15 advantages of the bca protein assay 16 disadvantages of the bca protein assay 17 bca, reducing agent compatible 18 bca protein assay 19 micro bca protein assay 20. The pierce bca protein assay is a detergentcompatible formulation based on bicinchoninic acid bca for the colorimetric detection and quantitation of total protein. Pierce bca reagents provide accurate determination of protein concentration with most sample.